高效液相色谱法同时测定氧化型染发产品中33种染发剂

建立了高效液相色谱同时测定氧化型染发产品中33种禁限用染发剂含量的分析方法。采用Waters Atlantis T3 MV Kit色谱柱(250 mm×4.6 mm, 5 μm),以磷酸盐缓冲液-乙腈为流动相,梯度洗脱,流速为1.0 mL/min ,柱温为25 ℃;采用二极管阵列检测器(DAD),检测波长为235及280 nm,进样量为5 μL。结果表明,各成分在线性范围内线性关系良好,相关系数均高于0.999,各成分精密度实验相对标准偏差(RSDs)均小于2%;四氨基嘧啶硫酸盐和2,4-二氨基苯氧基乙醇盐酸盐在12 h内稳定性实验RSDs小于5%,其余31种成分在24 h内稳定性实验RSDs均小于5%;各成分在3个浓度水平下的加标回收率为77.6%~116.3%。该方法简便、快速、准确,适用于氧化型染发产品中多种染发剂的检测。

Simultaneous determination of 33 hair dyes in oxidative hair dye products by high performance liquid chromatography

A method was developed for the simultaneous determination of 33 hair dyes in oxidative hair dye products by high-performance liquid chromatography (HPLC). The analytes were separated on a Waters Atlantis^® T3 MV Kit column (250 mm×4.6 mm, 5 μm) by gradient elution using phosphate buffer-acetonitrile as mobile phases. The components were detected at two different wavelengths of 235 and 280 nm using a diode array detector. The results showed that the method had good linearity with correlation coefficients exceeding 0.999. Precision was good for the 33 analytes, with the relative standard deviations (RSDs) less than 2%. Stabilities of tetraaminopyrimidine sulfate and 2,4-diaminophenoxyethanol HCl in 12 h, as well as other analytes in 24 h, were good, with RSDs less than 5%. Recoveries of the method ranged from 77.6% to 116.3% at three different concentration levels. The method proved to be simple, rapid, and accurate, suitable for the determination of various hair dyes in oxidative hair dye products