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Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata: Characterization and Antibacterial Activity Against Escherichia coli Isolated from Poultry Faeces |
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| Authors: | Chunchegowda Udayashankar Arakere Shivaram Ashwini Bagepalli Mahadevamurthy Murali Ramachndrappa Lakshmeesha Thimappa Lalitha Sreelatha Gopalakrishna Krishnappa Hemanth Kumar Naguvanahalli Anandan Satish Sudarshana Brijesh Singh Chanappa Eswaraiah Ganadhal Ramachandrappa Niranjana Siddapura |
| Institution: | 1.Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, 570 006, India ;2.Department of Microbiology, Sri Siddhartha Medical College, Tumakuru, Karnataka, 572107, India ;3.Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru, Karnataka, 570 006, India ;4.Department of Microbiology and Biotechnology, Jnanabharathi Campus, Bangalore University, Bengaluru, Karnataka, 560 056, India ;5.Department of Crop Protection, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, 695 017, India ;6.Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru, Karnataka, 570 006, India ;7.Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysuru, Karnataka, 570 006, India ;8.Government Science College, NT Road, Bengaluru, 560001, India ; |
| Abstract: | The current study was undertaken to investigate the antibacterial (against molecular characterized E. coli isolated from poultry faeces) potential of biosynthesized zinc oxide nanoparticles (ZnO-NPs) from Passiflora subpeltata Ortega aqueous leaf extract. The biosynthesized nanoparticles were subjected to physico-chemical characterization to study shape, size and purity by UV–Vis spectroscopy, X-Ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The molecular identification of isolated E. coli from faeces samples was carried out by using 16–23s rRNA primers. The results of the physico-chemical characterization revealed that the biosynthesized nanoparticles were of 93.7% purity with an average size between 45 and 50 nm. The ZnO-NPs offered significant inhibition against the isolated Gram-negative E. coli with MIC at 62.5 µg mL?1 concentration. The antibacterial potential of ZnO NPs against E. coli has also been investigated by the cell viability test, and further the effects of ZnO NPs on bacterial morphological structures was analysed by SEM and TEM. |
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