Simultaneous determination of aflatoxins B1, B2, G1, G2 in Fructus Bruceae by high‐performance liquid chromatography with online postcolumn photochemical derivatization |
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| Authors: | Jiliang Cao Shujun Zhou Weijun Kong Xiaochi Ma Meihua Yang Li Wan Shihai Yang |
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| Institution: | 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, , Beijing, China;2. School of Pharmacy, Chengdu University of TCM, , Chengdu, China;3. College of Chinese Medicinal Material, Jilin Agricultural University, , Changchun, China;4. College of Pharmacy, Dalian Medical University, , Dalian, China |
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| Abstract: | A simple, reliable, and highly sensitive method for the simultaneous determination of aflatoxin B1, B2, G1, G2 in Fructus Bruceae was developed using high‐performance liquid chromatography coupled to online postcolumn photochemical derivatization and fluorescence detection. Aflatoxins were first extracted by a methanol/water mixture and then cleaned up with an AflaTest? immunoaffinity column. Different clean‐up and derivatization methods were compared and optimized. The established method was extensively validated to show satisfactory performance of linearity (R2 ≥ 0.9997), recovery (74.3–100.8%), and precision (RSDs ≤ 2.8%) for the investigated aflatoxins. This proposed method was also applied to 11 F. Bruceae samples and the results showed that 10 out of 11 were contaminated with aflatoxins ranging from 0.26 to 27.52 μg/kg and the occurrence of aflatoxin B1, the most toxic one, was as high as 91% in all the samples, highlighting the severe contamination and the necessity to set legal limits for aflatoxins in F. Bruceae. |
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| Keywords: | Aflatoxins Fluorescence detection Fructus Bruceae Photochemical derivatization |
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