Development and testing of a CW-EPR apparatus for imaging of short-lifetime nitroxyl radicals in mouse head |
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| Authors: | Sato-Akaba Hideo Fujii Hirotada Hirata Hiroshi |
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| Affiliation: | aDepartment of Electrical Engineering, Yamagata University, Yonezawa, Yamagata 992-8510, Japan;bSchool of Health Sciences, Sapporo Medical University, Sapporo 060-8556, Japan;cGraduate Program of Human Sensing and Functional Sensor Engineering, Yamagata University, Yonezawa, Yamagata 992-8510, Japan |
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| Abstract: | ![]()
This article describes a method for reducing the acquisition time in three-dimensional (3D) continuous-wave electron paramagnetic resonance (CW-EPR) imaging. To visualize nitroxyl spin probes, which have a short lifetime in living organisms, the acquisition time for a data set of spectral projections should be shorter than the lifetime of the spin probes. To decrease the total time required for data acquisition, the duration of magnetic field scanning was reduced to 0.5 s. Moreover, the number of projections was decreased by using the concept of a uniform distribution. To demonstrate this faster data acquisition, two kinds of nitroxyl radicals with different decay rates were measured in mice. 3D EPR imaging of 4-hydroxy-2,2,6,6-tetramethylpiperidine-d17-1-15N-1-oxyl in mouse head was successfully carried out. 3D EPR imaging of nitroxyl spin probes with a half-life of a few minutes was achieved for the first time in live animals. |
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| Keywords: | 3D EPR imaging Uniform distribution of projections Nitroxyl radicals Geodesic sphere |
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