Simple high-performance liquid chromatographic method for the measurement of amiloride in body fluids |
| |
| Authors: | G Forrest G T McInnes A P Fairhead G G Thompson M J Brodie |
| |
| Affiliation: | University Department of Medicine, Gardiner Institute, Western Infirmary, Glasgow, Scotland, U.K. |
| |
| Abstract: | ![]()
A simplified rapid high-performance liquid chromatographic (HPLC) procedure has been developed for the measurement of amiloride in plasma or urine. Solid-phase extraction columns provide quick, clean and simple sample preparation, allowing ten samples to be processed in less than 5 min. The HPLC system uses a standard reversed-phase (C18) column with detection by ultraviolet absorption at 365 nm. The assay has been used to define plasma amiloride concentration-time profiles and to quantitate urine amiloride recovery in healthy men after repeated administration at two doses. |
| |
| Keywords: | |
|
|
