鹅去氧胆酸分子印迹聚合物微球的制备及选择性分子识别

以鹅去氧胆酸(CDCA)为印迹分子, 甲基丙烯酸为功能单体, 丙烯酸乙二醇二甲基酯和三羟甲基丙烷三甲基丙烯酸酯为交联剂, 在氯仿中采用沉淀聚合法制得平均粒径为200~300 nm的分子印迹聚合物微球(MIPMS). 用红外光谱研究了印迹分子与功能单体之间的作用类型, 用透射电镜对聚合物的形貌进行了表征. 结果表明, 聚合物微球在合成过程中形成了两类结合位点, 该分子印迹聚合物对CDCA具有良好的特异吸附性能, 可用于胆汁酸的分离、纯化, 交联剂的种类可以影响分子印迹聚合物的形貌和吸附性能.

Preparation of Molecularly Imprinted Polymer Microspheres and Their Recognition for Chenodeoxycholic Acid

Molecularly imprinted polymer microspheres were obtained by deposition polymerization with methyacrylic acid(MAA) as the functional monomer, trimethylolpropane trimethacrylate(TRIM) and ethylene glycoldimethacrylate(EDGMA) as the cross-linkers, chenodeoxycholic acid(CDCA) as the template molecule, and 2,2-azobis-isobutyronitrile as the initiation agent. The average size of the molecularly imprinted polymer microspheres was between 200 nm and 300 nm. The binding properties of the template molecule on MIPMS were evaluated by Scatchard analysis. The selective adsorptions of target molecule on MIPMS were studied. The results indicate that there were two class binding sites in polymers and the MIPMS showed a specific affinity toward chenodeoxycholic acid.