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Enantiomeric separation of various lipoxygenase derived monohydroxy polyunsaturated fatty acid methyl esters by high-performance liquid chromatography |
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| Authors: | D. Martini and G. Iacazio |
| Affiliation: | a ENSSPICAM, Laboratoire de Catalyse Asymétrique, Av. Escadrille Normandie Niemen, 13397 Marseille Cedex 20, France b Faculté des Sciences de St Jérôme, Laboratoire de Microbiologie, 13397 Marseille Cedex 20, France |
| Abstract: | Lipoxygenase derived monohydroxy polyunsaturated fatty acid methyl esters were separated by chiral high-performance liquid chromatography (HPLC) with a Chiralcel OD-H column in the normal-phase mode. Major lipoxygenase derivatives of linoleic, -linolenic and arachidonic acids are well resolved by this column, provided they have been individually purified. Our method allows an easy and rapid determination of lipoxygenases enantioselectivity. In all cases tested the R enantiomer is eluted first. |
| Keywords: | Fatty acid methyl esters Lipoxygenases |
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