Tunable mid-infrared,high-energy femtosecond laser source for glyco-protein structure analysis |
| |
| Authors: | T Imahoko K Takasago T Sumiyoshi H Sekita K Takahashi M Obara |
| |
| Institution: | (1) Cyber Laser Inc., Telecom Center Bldg., 2-38 Aomi, Koto-ku, Tokyo 135-8070, Japan;(2) Computational Biology Research Center, AIST Tokyo Waterfront, 2-41-6 Aomi, Koto-ku, Tokyo 135-0064, Japan;(3) School of Integrated Design Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama-shi 223-8522, Japan |
| |
| Abstract: | We have developed a 6–12 μm mid-infrared (MIR) femtosecond laser source for glyco-protein structure analysis. The MIR femtosecond
laser pulses are generated by a differential frequency generation (DFG) configuration with a combination of Ti:sapphire based
regeneratively amplified femtosecond laser pulses (780 nm, 160 fs, 1 mJ) and a β-BaB2O4 (BBO) based optical parametric amplifier (OPA). The MIR pulse energy exceeds 4.5 μJ, where a glyco-protein molecule has resonant
absorption lines due to the vibrational–rotational transitions. The pulse width is estimated to be less than 1 ps according
to the cross correlation measurement between the two OPA output pulses. Using the MIR femtosecond laser pulses, we demonstrated
photo-dissociation of the sialyl Lewis X (sLeX) proton added ion, which is the first time to the best of our knowledge.
PACS 42.65.Re; 42.62.-b; 42.60.-b; 42.65.-k; 87.50 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
