A simple visual method for DNA detection based on the formation of gold nanoparticles |
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| Institution: | 1. State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;2. Hunan Provincial Key Lab of Dark Tea and Jin-Hua, School of Materials and Chemical Engineering, Hunan City University, Yiyang 413000, China;3. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China;4. Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China;5. National Center for International Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Theranostics, Guangxi Medical University, Nanning 530021, China;1. State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;2. Department of Chemistry, University of Michigan, Ann Arbor MI 48109, United States;3. Department of Orthopedic, Nanjing Jinling Hospital, Nanjing 210096, China;1. Grup de Biotecnologia Molecular i Industrial, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Rambla Sant Nebridi 22, Terrassa 08222, Spain;2. Unitat de ferides Complexes, Consorci Sanitari de Terrassa Hospital de Terrassa, Ctra. Torrebonica, s/n, 08227 Terrassa, Barcelona, Spain |
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| Abstract: | A simple visual method for DNA detection during the formation of gold nanoparticles (AuNPs) was developed based on different electrostatic properties of single strand DNA (ssDNA) and double strand DNA (dsDNA). Since the ssDNA is easy to bind to AuNPs due to its exposed bases which could prevent salt-induced aggregation of AuNPs. The dsDNA always present negative charge because its negatively charged phosphate backbone is exposed. In this case, the dsDNA could disturb the adsorption between dsDNA and AuNPs and result in non-aggregation of AuNPs. After hybridization, chloroauric acid and ascorbic acid were added to the mixture solution, and the solution changed to red immediately and turned to purple in 10 min in the present of target DNA. TEM results confirmed that the change of color stemed from aggregation of AuNPs. In order to obtain accurate results by naked eye, the DNA detection assay should be conducted under pH 7.0. |
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| Keywords: | Visual method Unmodified AuNPs DNA Hybridization adsorption Detection |
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