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Laser-induced fluorescence microscopic system using an optical parametric oscillator for tunable detection in microchip analysis |
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| Authors: | |
| Institution: | (1) Department of Ecosystem Engineering, Graduate School of Engineering, University of Tokushima, 2-1 Minami-jousanjima, Tokushima 770-8506, Japan;(2) Present address: Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan;(3) Present address: Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu Gunma 376-8515, Japan |
| Abstract: | A laser-induced fluorescence microscopic system based on optical parametric oscillation has been constructed as a tunable detector for microchip analysis. The detection limit of sulforhodamine B (Ex. 520 nm, Em. 570 nm) was 0.2  mol, which was approximately eight orders of magnitude better than with a conventional fluorophotometer. The system was applied to the determination of fluorescence-labeled DNA (Ex. 494 nm, Em. 519 nm) in a microchannel and the detection limit reached a single molecule. These results showed the feasibility of this system as a highly sensitive and tunable fluorescence detector for microchip analysis. |
| Keywords: | Optical parametric oscillation Microchip Laser-induced fluorescence Microscopy Sulforhodamine B Tunable |
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