Stimulated Production of Triterpenoids of <Emphasis Type="Italic">Ganoderma lucidum</Emphasis> by an Ether Extract from the Medicinal Insect, <Emphasis Type="Italic">Catharsius molossus</Emphasis>, and Identification of the Key Stimulating Active Components |
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Authors: | Gao-Qiang Liu Hua-Xi Xiao Xiao-Ling Wang Yao Zhao Yong-Guang Zhang Guo-Pu Ren |
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Institution: | (1) Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, 410004 Changsha, China;(2) National Engineering Laboratory for Grain Further Processing, Central South University of Forestry and Technology, 498 South Shaoshan Road, 410004 Changsha, China;(3) Hunan AVA Dairy Co., Ltd., 108 Leifengdadao Road, 410004 Changsha, China |
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Abstract: | The medicinal fungus Ganoderma lucidum was inoculated into the media with and without supplementation of medicinal insect extracts to screen stimulators from Chinese
medicinal insects for mycelial growth and triterpenoids production in submerged fermentation. The methanol and ether extracts
of the tested insects had no significant stimulatory effect on the mycelial biomass production (P > 0.05), and those of H. remigator and Mylabris phalerata markedly inhibited the mycelial growth. However, the ether extract of Catharsius molossus at a concentration of 200 mg l−1 led to a significant increase in triterpenoids concentration from 231.7 ± 9.77 to 313.7 ± 10.6 mg l−1 (P < 0.01). Analysis of fermentation kinetics of G. lucidum suggests that glucose concentration in the extract of C. molossus-added group decreased more quickly as compared to the control group from day 2 to day 7 of fermentation process, while the
triterpenoids biosynthesis was promoted at the same culture period. However, the culture pH profile was not affected by the
addition of the extract. Chemical study of the extract show that cis-9,10-methylenehexadecanoic acid (9,10-MEA) and hexadecanoic acid (especially 9,10-MEA) were the key active compounds of the
extract responsible for the stimulatory effect on the triterpenoids production. |
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