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富硒蛹虫草试样中硒的形态分析
引用本文:邓桂春,侯松嵋,铁梅,张新,孟伟,于桂英,臧树良.富硒蛹虫草试样中硒的形态分析[J].分析科学学报,2006,22(1):21-24.
作者姓名:邓桂春  侯松嵋  铁梅  张新  孟伟  于桂英  臧树良
作者单位:辽宁大学化学系,沈阳,110036;辽宁大学化学系,沈阳,110036;中国科学院沈阳应用生态研究所陆地生态过程重点实验室,沈阳,110036;辽宁大学化学系,沈阳,110036;华东师范大学化学系,上海,200062
摘    要:本文采用连续浸提法研究了富硒蛹虫草中硒的赋存形态。结果表明,碱溶态>盐溶态>水溶态>醇溶态>残渣态。可见,碱浸取是获取植物蛋白的有效途径,在富硒蛹虫草试样中硒主要以硒蛋白的形式存在。

关 键 词:富硒蛹虫草  形态分析  分子荧光  连续浸取
文章编号:1006-6144(2006)01-0021-04
收稿时间:2004-09-22
修稿时间:2004-11-02

Speciation Analysis of Selenium in Cordyceps Militaris by Fluorescence Spectrophotometry
DENG Gui-chun,HOU Song-mei,TIE Mei,ZHANG Xin,MENG Wei,YV Gui-ying,ZANG Shu-liang.Speciation Analysis of Selenium in Cordyceps Militaris by Fluorescence Spectrophotometry[J].Journal of Analytical Science,2006,22(1):21-24.
Authors:DENG Gui-chun  HOU Song-mei  TIE Mei  ZHANG Xin  MENG Wei  YV Gui-ying  ZANG Shu-liang
Institution:1. Department of Chemistry ,Liaoning University, Shenyang 110036; 2. Department of Chemistry, East China Normal University, Shanghai 200062 ; 3. Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology Chinese Academy of Sciences, Shenyang 110036
Abstract:The sequential chemical extractions have been developed for partitioning selenium in Cordyceps militaris into five fractions:ethanol soluble,water soluble,salt soluble,KOH solution saluble and residual.The sum of selenium in the fractions from high to low is in the order of KOH solution saluble> salt soluble> water soluble> ethanol soluble> residual.Alkali extraction is the most effective way to obtain protein from plant,and the anount of selenium in alkoline solution is the highest,so it can be concluded that the major existing form of selenium in Cordyceps militaris is selenium protein.
Keywords:Enricjed-selenium Cordyceps militaris  Speciation  Fluorescence spectrophotometry  Sequential extraction
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