Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface |
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Authors: | Anna Laura Capriotti Giulio Caracciolo Chiara Cavaliere Carlo Crescenzi Daniela Pozzi Aldo Laganà |
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Institution: | 1.Dipartimento di Chimica,Sapienza Università di Roma,Rome,Italy;2.Dipartimento di Medicina Molecolare,Sapienza Università di Roma,Rome,Italy;3.Dipartimento di Scienze Farmaceutiche e Biomediche,Università di Salerno,Fisciano (SA),Italy |
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Abstract: | The knowledge about the interaction between plasma proteins and nanocarriers employed for in vivo delivery is fundamental
to understand their biodistribution. Protein adsorption onto nanoparticle surface (protein corona) is strongly affected by
vector surface characteristics. In general, the primary interaction is thought to be electrostatic, thus surface charge of
carrier is supposed to play a central role in protein adsorption. Because protein corona composition can be critical in modifying
the interactive surface that is recognized by cells, characterizing its formation onto lipid particles may serve as a fundamental
predictive model for the in vivo efficiency of a lipidic vector. In the present work, protein coronas adsorbed onto three
differently charged cationic liposome formulations were compared by a shotgun proteomic approach based on nano-liquid chromatography–high-resolution
mass spectrometry. About 130 proteins were identified in each corona, with only small differences between the different cationic
liposome formulations. However, this study could be useful for the future controlled design of colloidal drug carriers and
possibly in the controlled creation of biocompatible surfaces of other devices that come into contact with proteins into body
fluids. |
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