Novel amphiphilic fluorescent graft copolymers: synthesis,characterization, and potential as gene carriers |
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| Authors: | Zhaoqiang Wu Lingzhi Meng |
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| Institution: | 1. College of Chemistry and Material Sciences, South‐Central University for Nationalities, Wuhan 430074, P.R. ChinaCollege of Chemistry and Material Sciences, South‐Central University for Nationalities, Wuhan 430074, P.R. China.;2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P.R. China |
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| Abstract: | Amphiphilic fluorescent graft copolymer (PVP‐PyATAm) was successfully synthesized by the free radical copolymerizations of hydrophobic monomer N‐acryloyl‐thioureylene‐4‐(1‐pyrene)‐butyryl amide (PyATAm) with hydrophilic precursor polymers of vinyl‐functionalized poly (N‐vinylpyrrolidone) (Acryloyl‐PVP) in DMF. FT‐IR, 1H NMR, TEM, gel permeation chromatography‐multi‐angle laser light scattering, UV‐vis spectroscopy, viscometric measurement, and fluorescence spectroscopy were used to characterize this copolymer. The TEM observation showed that the copolymer PVP‐ PyATAm formed spherical micelles in an aqueous solution and the size of micelles was between 50 and 70 nm in diameter. The interaction of PVP‐PyATAm copolymer and plasmid DNA was examined by agarose gel electrophoresis and TEM. Results indicated that the copolymer–DNA complexes were self‐assembled and the size of complexes was between 90 and 120 nm in diameter. Cytotoxity studies using MTT colorimetric assays suggested good biocompatibility of PVP‐PyATAm in vitro. These results suggested the potential of this graft copolymer as gene delivery carrier. Copyright © 2007 John Wiley & Sons, Ltd. |
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| Keywords: | amphiphilic copolymer poly (N‐vinylpyrrolidone) gene delivery fluorescence DNA |
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