Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dismutase and hCuZnSOD in Pichiapastoris |
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| Authors: | QU He-zhi DU Shan-shan YANG Shuo HUANG Lu ZHANG Lei XIAO Song HAO Dong-yun WANG Xiao-ping |
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| Institution: | [1]Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin Unversity, Changchun 130021, P. R. China [2]Biotechnology Research Centre, Jilin Academy of Agricultural Science, Changchun 130033, P. R. China |
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| Abstract: | Mammalian cells express two isoforms of Cu-and Zn-containing superoxide dismutases(SODs),CuZnSOD and extracellular SOD(EC-SOD),involved in the defense system against reactive oxygen species(ROS).The two SODs have structurally homologous centre domain with distinct N-and C-terminuses,resulting in the different characteristics of the structure and function of the two molecules.We generated a hybrid SOD molecule(namely hySOD) via replacing the N-and C-terminuses of hCuZnSOD with the counterparts of hEC-SOD.The... |
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| Keywords: | Extracellular superoxide dismutase Protein fusion Heterologous expression Pichia pastoris |
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