Simultaneous determination of eleven amphenicols and antiviral drugs in aquatic products by ultra-high performance liquid chromatography-tandem mass spectrometry coupled with PRIME HLB solid phase extractionEI北大核心CSCD

An efficient ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of 11 amphenicols and antiviral drugs in aquatic products including fish, shrimp, soft-shell turtle and shellfish samples. Each sample was dispersed with 4%(w/w)sodium chloride solution, and extracted with 2%(V/V) ammonia-ethyl acetate solution. After the concentration of the extract, the residue was redissolved in 0.2%(V/V) aqueous solution of formic acid-acetonitrile (20∶80, V∶V), purified with Oasis PRIME HLB cartridge, and detected by UPLC-MS/MS. The samples were analyzed directly on the ZORBAX RRHD Eclipse Plus C18 column using 5 mmol/L ammonium formate and acetonitrile as mobile phase. The quantitative determination of the analytes was carried out under the multiple reaction monitoring mode with positive and negative electrospray ionization using internal standard method. Results showed that there were good linear relationships for all analytes in the corresponding concentration ranges, with the correlation coefficients not less than 0.996. The limits of quantification ranged from 0.2 to 2.0 µg/kg. The average spiked recoveries were between 84.6% and 110% with the relative standard deviations of 1.0% to 14%. The method, which has been used in risk monitoring, is suitable for rapid determination of amphenicols and antiviral drugs in aquatic products. © 2023, Youke Publishing Co.,Ltd. All rights reserved.