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Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection
引用本文:HuaZHANG WenRuiJIN. Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection[J]. 中国化学快报, 2003, 14(9): 952-954
作者姓名:HuaZHANG WenRuiJIN
作者单位:[1]StateKeyLaboratoryofMicrobialTechnology,ShandongUniversity,Jinan250100 [2]SchoolofChemistry-andChemicalEngineering,ShandongUniversity,Jinan250100
摘    要:
A novel approach for analysis of amino acids in individual erythrocytes was established.In this method, the derivatization reagent was in~oduced into the living cells by electroporation.After derivatization, the amino acids in a single cell were determined by capillary electrophoresis with laser-induced fluorescence detection.

关 键 词:氨基酸 红血球 毛细管电泳 FITC-衍生化作用 激光感应荧光检测 异硫氰酸盐 单细胞分析

Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection
Hua Zhang,Wen Rui JIN. Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection[J]. Chinese Chemical Letters, 2003, 14(9): 952-954
Authors:Hua Zhang  Wen Rui JIN
Abstract:
A novel approach for analysis of amino acids in individual erythrocytes was established. In this method, the derivatization reagent was introduced into the living cells by electroporation. After derivatization, the amino acids in a single cell were determined by capillary electrophoresis with laser-induced fluorescence detection.
Keywords:Capillary electrophoresis   laser-induced fluorescence detection   single cell analysis   amino acid.
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