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日落黄与牛血清白蛋白相互作用的电化学研究
引用本文:李金凰,陈毅挺,郑晶晶,潘彦.日落黄与牛血清白蛋白相互作用的电化学研究[J].分析测试技术与仪器,2017,23(1):6-10.
作者姓名:李金凰  陈毅挺  郑晶晶  潘彦
作者单位:1. 闽江学院化学与化学工程系,福建福州,350108;2. 闽江学院化学与化学工程系,福建福州350108;绿色染整福建省高校工程研究中心,福建福州350108
基金项目:国家自然科学基金(21405075);福建省工业科技计划重点项目(2014H0040);福建省高校青年自然基金重点项目(JZ160468);国家级大学生创新创业项目(201610395009);闽江学院科技项目(MYK15001)资助
摘    要:利用微分脉冲伏安法,研究了日落黄与牛血清白蛋白之间的相互作用.试验结果表明,日落黄与牛血清白蛋白之间形成近似1:1的复合物,从而造成日落黄峰电流的降低,两者之间的结合主要依靠静电力,且结合常数随温度升高而降低.研究有利于从分子水平上了解日落黄和牛血清白蛋白之间的结合作用,为更进一步了解日落黄的生物代谢机理提供一定的理论参考.

关 键 词:日落黄  牛血清白蛋白  电化学
收稿时间:2016/12/13 0:00:00
修稿时间:2017/2/22 0:00:00

Electrochemical Studies on Interaction between Sunset Yellow and Bovine Serum Albumin
LI Jin-huang,CHEN Yi-ting,ZHENG Jing-jing and PAN Yan.Electrochemical Studies on Interaction between Sunset Yellow and Bovine Serum Albumin[J].Analysis and Testing Technology and Instruments,2017,23(1):6-10.
Authors:LI Jin-huang  CHEN Yi-ting  ZHENG Jing-jing and PAN Yan
Institution:Chemistry and Chemical Engineering Department of Minjiang University, Fuzhou 350108, China;Chemistry and Chemical Engineering Department of Minjiang University, Fuzhou 350108, China;Enngineering Research Center of Green Dyeing and Finishing in Fujian Provincial University, Fuzhou 350108, China;Chemistry and Chemical Engineering Department of Minjiang University, Fuzhou 350108, China;Chemistry and Chemical Engineering Department of Minjiang University, Fuzhou 350108, China
Abstract:The interaction between sunset yellow (SY) and bovine serum albumin (BSA) was studied by differential pulse voltammetry. The results showed that the decrease of peak current was caused by the formation of SY and BSA (approximately, 1:1)composite. The electrostatic force played the main role in their combination. The binding constant was decreased with increasing temperature. The research results are helpful in understanding the metabolism mechanism of SY and BSA at a molecular level, and provide some theoretical references for the study of the biological metabolism mechanism of SY.
Keywords:sunset yellow  bovine serum albumin  electrochemical
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