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Role of the solvent in thin-layer chromatography of steroids on alumina |
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| Authors: | S M Petrovi? L A Kolarov Julijana A Petrovi? |
| Institution: | (1) Institute of Microbiological Processes and Applied Chemistry, Faculty of Technology, University of Novi Sad, 21000 Novi Sad, Yugoslavia;(2) Institute of Chemistry, Faculty of Sciences, University of Novi Sad, 21000 Novi Sad, Yugoslavia |
| Abstract: | The effects of different binary solvents on the retention behaviour of some steroids in thin-layer chromatography on alumina were studied. The slope of the linear relationship between the retention constant of the steroid and the logarithm of the volume fraction fo the polar component in the binary solvent mixture depends predominantly on the diluent. Linear relationships between the axis intercepts and slopes of particular steroids exists for all chromatographic systems examined. The slope of this relationships is a function of the retention constant of the steroid hydroxyl group. |
| Keywords: | Thin-layer chromatography Alumina Retention relationships Steroids |
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