| In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane |
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| 作者姓名： | NongYueHE JianXinTANG SongLI HongCHEN AnCunZHOU |
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| 作者单位： | [1]LaboratoryofNanotechnology,BiomedicineandDevices,ZhuzhouInstituteofTechnology,Zhuzhou412008 [2]LaboratoryofNanotechnology,BiomedicineandDevices,ZhuzhouInstituteofTechnology,Zhuzhou412008／／Chien-ShiungWuLaboratory,SoutheastUniversity,Nanjing210096 [3]LaboratoryofNanotechnology,BiomedicineandDevices,ZhuzhouInstituteofTechnology,Zhuzhou412008／／LifeScienceandTechnologyDepartmentofCentralSouthForestryUniversity,Changsha410004 [4]CollegeofHunanTraditionalChineseMedicine,Zhuzhou412000 |
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| 摘 要： | Polypropylene microporous membranes were treated with plasma in a mixture of N2 and H2 (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstrated the success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm^2. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.
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| 关 键 词： | 本位合成 低聚核苷酸 等离子体 聚丙烯微孔膜 氨基团 |
In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane |
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| NongYueHE JianXinTANG SongLI HongCHEN AnCunZHOU.In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J].Chinese Chemical Letters,2005,16(3):397-400. |
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| Authors: | Nong Yue HE Jian Xin TANG Song LI Hong CHEN An Cun ZHOU Laboratory of Nanotechnology Biomedicine and Devices Zhuzhou Institute of Technology Zhuzhou Chien-Shiung Wu Laboratory Southeast University Nanjing Life Science and Technology |
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| Institution: | Nong Yue HE1 *,Jian Xin TANG1,Song LI1,Hong CHEN1,3,An Cun ZHOU4 2 1 Laboratory of Nanotechnology,Biomedicine and Devices,Zhuzhou Institute of Technology,Zhuzhou 412008 2Chien-Shiung Wu Laboratory,Southeast University,Nanjing 210096 3Life Science and Technology Department of Central South Forestry University,Changsha 410004 4College of Hunan Traditional Chinese Medicine,Zhuzhou 412000 |
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| Abstract: | |
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| Keywords: | Plasma grafting amino group in situ synthesis of oligonucleotides coupling efficiency polypropylene microporous membrane |
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