| 高效液相色谱法测定旋复花中槲皮素的含量 |
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| 引用本文： | 杨玲娟,焦成瑾,葛勐,雍宝霞. 高效液相色谱法测定旋复花中槲皮素的含量[J]. 化学分析计量, 2010, 19(1): 65-66 |
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| 作者姓名： | 杨玲娟 焦成瑾 葛勐 雍宝霞 |
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| 作者单位： | 天水师范学院生命科学与化学学院,天水,741001;天水师范学院生命科学与化学学院,天水,741001;天水师范学院生命科学与化学学院,天水,741001;天水师范学院生命科学与化学学院,天水,741001 |
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| 摘 要： | 建立了高效液相色谱法测定旋复花中槲皮素含量的方法。该方法采用ZORBAX eclipse XDB C8色谱柱(150mm×4.6mm,5μm),流动相为甲醇-磷酸(0.2%),体积比为65∶35,检测波长为370nm。实验结果表明,槲皮素在5.0～100.0μg/mL范围内与峰面积线性关系良好(r=0.9999),测定结果的相对标准偏差为1.2%(n=6),平均加标回收率为97.1%。该法可用于测定旋复花中槲皮素的含量。
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| 关 键 词： | 高效液相色谱法 槲皮素 旋复花 |
DETERMINATION OF QUERCETIN IN INULA BY HPLC |
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| Yang Lingjuan,Jiao Chengjin,Ge Meng,Yong Baoxia. DETERMINATION OF QUERCETIN IN INULA BY HPLC[J]. Chemical Analysis And Meterage, 2010, 19(1): 65-66 |
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| Authors: | Yang Lingjuan Jiao Chengjin Ge Meng Yong Baoxia |
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| Affiliation: | ( Colloge of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China) |
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| Abstract: | A method for determining quereetin in inula by HPLC was established. The analysis was performed on ZORBAX eclipse XDB Ca column （ 150 mm ×4.6 mm ,5 μm） at the detection wave length of 370 nm. The mobile phase was consisted of methanol -0.2% phosphoric acid （volume ratio was 65：35 ） with a flow rate of 1.0 mL/min. The calibration curve was linear in the range of 5.0 - 100.0 μ/mL of quercetin （ r = 0.999 9 ）. The RSD of determination results was 1.2% （ n = 6）, and the average recovery was 97.1%. The method is suitable for determination of quereetin in inula. |
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| Keywords: | HPLC quercetin inula |
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