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| Chemical Modification of Tryptophan Residues in Pullulanase | |
| 引用本文： | Li Rong TENG Hao FAN Yuan Yuan ZHANG Qi YU Yue Feng HUANG Lan Ying LIU. Chemical Modification of Tryptophan Residues in Pullulanase[J]. 中国化学快报, 2005, 16(10): 1335-1336 |
| 作者姓名： | Li Rong TENG Hao FAN Yuan Yuan ZHANG Qi YU Yue Feng HUANG Lan Ying LIU |
| 作者单位： | College of Life Science, Jilin University, Changchun 130023 |
| 摘 要： | Tryptophan（Trp）residues in pullulanase have been chemically modified with N-bromossuccinimide（NBS）. The results of ultraviolet spectra indicated that there are 18 Trp residues in pullulanase and nine of them are located on the surface of the enzyme. Three of these Trp residues are none-essential residues which showed the fastest reaction speed by Zhou＇s plot. Two of the seven relative faster reacting residues are essential for the activity of the enzyme. The other eight are none-reactive residues with lowest reaction speed. |
| 关 键 词： | 色氨酸 化学变化 紫外线 酶 |
| 收稿时间： | 2004-12-29 |
Chemical Modification of Tryptophan Residues in Pullulanase |
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| Li Rong TENG,Hao FAN,Yuan Yuan ZHANG,Qi YU,Yue Feng HUANG,Lan Ying LIU. Chemical Modification of Tryptophan Residues in Pullulanase[J]. Chinese Chemical Letters, 2005, 16(10): 1335-1336 | |
| Authors: | Li Rong TENG Hao FAN Yuan Yuan ZHANG Qi YU Yue Feng HUANG Lan Ying LIU |
| Abstract: | Tryptophan(Trp)residues in pullulanase have been chemically modified with N-bromossuccinimide(NBS). The results of ultraviolet spectra indicated that there are 18 Trp residues in pullulanase and nine of them are located on the surface of the enzyme. Three of these Trp residues are none-essential residues which showed the fastest reaction speed by Zhou's plot. Two of the seven relative faster reacting residues are essential for the activity of the enzyme. The other eight are none-reactive residues with lowest reaction speed. |
| Keywords: | Pullulanase tryptophan(Trp) chemical modification |
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