Measurement of longitudinal relaxation times in crowded 1H NMR spectra using one- and two-dimensional maximum quantum (MAXY) NMR spectroscopy |
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| Authors: | MAILI LIU CHAOHUI YE R. DUNCAN FARRANT JEREMY K. NICHOLSON JOHN C. LINDON |
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| Affiliation: | 1. Laboratories of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences , Wuhan, 430071, PR China;2. Physical Sciences Unit, Glaxo SmithKline Medicines Research Centre , Gunnels Wood Road, Stevenage, SG1 2NY, UK;3. Biological Chemistry, Biomedical Sciences Division, Imperial College of Science, Technology and Medicine , Sir Alexander Fleming Building, South Kensington, London, SW7 2AZ, UK |
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| Abstract: | ![]()
Methods for measuring longitudinal relaxation times of protons in heavily overlapped 1H NMR spectra are introduced and exemplified using a solution of cholesteryl acetate. The methods are based on 1-dimensional and 2-dimensional maximum quantum NMR spectroscopy (MAXY), which makes possible the selective detection of CH, CH2 and CH3 1H NMR resonances. A modification of the BIRD pulse sequence to achieve selective inversion of protons bonded to either 12C or 13C is given. The approach should find application in studies of molecular dynamics where isotopic enrichment is not possible and the level of available sample dictates the use of 1H NMR spectroscopy. |
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