Quick method for separating target compounds from the bark of Maqian (Zanthoxylum myriacanthum var. pubescens) by high‐performance countercurrent chromatography |
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| Authors: | Qingfei Fan Huanli Zhang Huabin Hu Youkai Xu Qishi Song |
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| Institution: | 1. Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China;2. Department of Biology University of Chinese Academy of Sciences, Beijing, P. R. China |
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| Abstract: | Choosing a suitable solvent system for a countercurrent chromatography separation presents a challenge for many researchers. In this study, we introduce a quick method of separating a target compound from the bark of Zanthoxylum myriacanthum var. pubescens by countercurrent chromatography. This method relies on the thin‐layer chromatography based generally useful estimation of solvent systems. This paper will present how to quickly choose a suitable solvent system with a thin‐layer chromatography based generally useful estimation of solvent systems working chart. O‐Methyltembamide ( 1 ) was enriched by countercurrent chromatography using n‐hexane/ethyl acetate/methanol/water (6:4:6:4) as the solvent system. Further purification was achieved by high‐performance liquid chromatography with purities of 98.2% from Z. myriacanthum var. pubescens bark. |
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| Keywords: | High‐performance countercurrent chromatography O‐Methyltembamide Zanthoxylum myriacanthum var pubescens |
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