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| 光谱法研究细胞色素c氧化酶CuA结构域蛋白的稳定性 | |
| 引用本文： | 李连之,宋爱新,黄仲贤. 光谱法研究细胞色素c氧化酶CuA结构域蛋白的稳定性[J]. 光谱实验室, 2004, 21(1): 135-137 |
| 作者姓名： | 李连之 宋爱新 黄仲贤 |
| 作者单位： | 聊城大学化学化工学院,山东省,聊城市,252059;复旦大学化学系化学生物学实验室,上海市,200433 |
| 基金项目： | 国家自然科学基金重大资助项目(No.39990600) |
| 摘 要： | 利用荧光光谱和圆二色谱研究了细胞色素c氧化酶CuA结构域蛋白的稳定性.结果表明,随着溶液pH值的升高,CuA结构域蛋白的稳定性降低.随着温度的升高,CuA结构域蛋白的二级结构遭到破坏.脱金属形式蛋白的热稳定性减小,表明双核金属铜中心的存在对CuA结构域蛋白的稳定性起重要作用. |
| 关 键 词： | 细胞色素c氧化酶 CuA结构域蛋白 荧光光谱 圆二色谱 |
| 文章编号： | 1004-8138(2004)01-0135-03 |
| 修稿时间： | 2003-07-01 |
Studies on the Stability of CUA Domain of Cytochrome c Oxidase by Spectral Methods |
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| LI Lian-Zhi. Studies on the Stability of CUA Domain of Cytochrome c Oxidase by Spectral Methods[J]. Chinese Journal of Spectroscopy Laboratory, 2004, 21(1): 135-137 | |
| Authors: | LI Lian-Zhi |
| Abstract: | The stability of Cu A domain of cytochrome c oxidase was investigated by fluorescence spectra and circular dichroism spectra.The results showed that the stability of Cu A domain decreased with the increasing of pH,and its secondary structure was destroyed with the increase of temperature.The thermal stability of apo-Cu A domain is higher than that of Cu A domain, which suggested that the binuclear metal center [Cu A +1.5-Cu A +1.5] also plays an important role on the structural stability of the protein. |
| Keywords: | Cytochrome c Oxidase Cu A Domain Fluorescence Spectra Circular Dichroism Spectra. |
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