Influence of amino acid side chain packing on Fe-methionine coordination in thermostable cytochrome C |
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| Authors: | Yamamoto Yasuhiko Terui Norifumi Tachiiri Naoki Minakawa Kazuhisa Matsuo Hitomi Kameda Tsunenori Hasegawa Jun Sambongi Yoshihiro Uchiyama Susumu Kobayashi Yuji Igarashi Yasuo |
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| Affiliation: | Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan. yamamoto@staff.chem.tsukuba.ac.jp |
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| Abstract: | ![]()
Paramagnetic NMR and optical studies of the oxidized forms of mesophile Pseudomonas aeruginosa cytochrome c(551) and its quintuple mutant (F7A/V13M/F34Y/E43Y/V78I), and thermophile Hydrogenobacter thermophilus cytochrome c(552) demonstrated that the amino acid side chain packings in the protein interior influence the coordination bond between the heme iron and the axial methionine in the proteins. The strength of heme axial coordinations was found to correlate with the overall protein thermostability. |
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