Finding of Optimal Calcium Ion Probes for Fluorescence Lifetime Measurement |
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| Authors: | Keisuke Yoshiki Hiroki Azuma Kazuhiko Yoshioka Mamoru Hashimoto Tsutomu Araki |
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| Affiliation: | (1) Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan |
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| Abstract: | We have investigated the fluorescence lifetime properties of 8 calcium ion probes, calcium-green-1, calcium green-2, calcium green-5N, calcium orange, oregon green 488 BAPTA-6F, fluo-3, fluo-4, and fluo-5N. We found that the decay time of calcium green-5N varied more sensitively with calcium concentration than calcium green-1 which was known to be a highly sensitive probe. We have also found that the center of observable range of calcium concentration by fluorescence lifetime measurement is lower than that by fluorescence intensity measurement. |
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| Keywords: | fluorescence lifetime calcium concentration calcium green-1 oregon green 488 BAPTA-6F calcium green-5N fluo-3 fluo-4 fluo-5N |
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