| 流动注射化学发光法灵敏检测牛血清白蛋白 |
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| 引用本文： | 杜俊俊,王辉,任书伟,周敏,刘迎迎,刘彦明. 流动注射化学发光法灵敏检测牛血清白蛋白[J]. 光谱学与光谱分析, 2013, 33(6): 1693-1696. DOI: 10.3964/j.issn.1000-0593(2013)06-1693-04 |
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| 作者姓名： | 杜俊俊 王辉 任书伟 周敏 刘迎迎 刘彦明 |
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| 作者单位： | 1. 信阳师范学院化学化工学院，河南 信阳 464000
2. 信阳市中心医院，河南 信阳 464000 |
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| 基金项目： | 国家自然科学基金项目，河南省高校创新人才工程项目 |
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| 摘 要： | 基于牛血清白蛋白(BSA)在碱性介质中对luminol-H2O2体系化学发光的增敏作用，结合流动注射化学发光技术提出了一种快速、 灵敏检测BSA的新方法。在优化条件下，检测BSA的线性范围为2.5×10-9～1.0×10-6 mol·L-1，检出限为5.8×10-10 mol·L-1，样品检测速率达112个·h-1。对1.0×10-7 mol·L-1 BSA溶液重复测定11次，相对标准偏差RSD为0.8%。该方法已成功应用于实际样品牛血清中BSA含量的测定。结合化学发光光谱和紫外可见吸收光谱，对luminol-H2O2-BSA体系可能的反应机理进行了探讨。
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| 关 键 词： | 流动注射 化学发光 牛血清白蛋白 反应机理 |
| 收稿时间： | 2012-09-14 |
Sensitive Determination of Bovine Serum Albumin by Flow-Injection Chemiluminescence |
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| DU Jun-jun , WANG Hui , REN Shu-wei , ZHOU Min , LIU Ying-ying , LIU Yan-ming. Sensitive Determination of Bovine Serum Albumin by Flow-Injection Chemiluminescence[J]. Spectroscopy and Spectral Analysis, 2013, 33(6): 1693-1696. DOI: 10.3964/j.issn.1000-0593(2013)06-1693-04 |
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| Authors: | DU Jun-jun WANG Hui REN Shu-wei ZHOU Min LIU Ying-ying LIU Yan-ming |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China2. Xinyang Central Hospital, Xinyang 464000, China |
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| Abstract: | A new, rapid and sensitive method for the determination of bovine serum albumin (BSA) by flow-injection coupled with chemiLuminescence detection was establish, which based on BSA enhancing effect on weak chemiLuminescence system of Luminol-H2O2 in alkaline solution. Under the optimized conditions, linearity for BSA was found in the range of 2.5×10-9～1.0×10-6 mol·L-1, with the detection limit of 5.8×10-10 mol·L-1. And the sampling detecting rate was about 112 samples·h-1. The relative standard deviation(RSD)was found to be 0.8% for 11 replicate determinations of 1.0×10-7 mol·L-1 BSA. The proposed method has been satisfactorily applied to the determination of BSA in the bovine serum samples. The possible mechanism of Luminol-H2O2-BSA was studied by chemiLuminescence spectrum and UV-visible spectrum. |
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| Keywords: | Flow-injection ChemiLuminescence Bovine serum albumin Mechanism |
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