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PHOTOSENSITIZATION OF HUMAN DIPLOID CELL CULTURES BY INTRACELLULAR FLAVINS AND PROTECTION BY ANTIOXIDANTS |
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| Authors: | Olivia M. Pereira James R. Smith Lester Packer |
| Affiliation: | Membrane Bioenergetics Group, Energy and Environment Division, Lawrence Berkeley Laboratory and Department of Physiology-Anatomy, University of California, Berkeley, CA 94720, and the Physiology Research Laboratory, Veterans Administration Hospital, Martinez, CA 94553, U.S.A. |
| Abstract: | Abstract— The damaging effects of near ultraviolet and visible light on WI-38 human diploid lung fibroblasts were investigated. WI-38 cells in culture were killed by light doses ranging from 2 to 10 × 10 Flavins were clearly implicated as endogenous photosensitizers, and antioxidants such as d, l -α-tocopherol (vitamin E), BHT and ascorbic acid were found to afford the cells protection from light damage. Furthermore, products of lipid peroxidation could be detected in cell homogenates irradiated in the presence of ribofiavin. |
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