紫外可见分光光度计的FDA仪器认证方法探讨

介绍紫外可见分光光度计符合FDA仪器性能认证的方法。主要对灯能量、吸光度的准确度和仪器线性等检定规程中没有或者确认方法不同的项目进行性能确认。分别在235,257,313,350 nm处,对重铬酸钾标准溶液进行测定,若吸收系数分别在123.0~126.0,142.8~146.2,47.0~50.3,105.5~108.5范围内,则仪器符合FAD认证要求;对系列重铬酸钾标准溶液进行测定,若吸光度与浓度的相关系数r2≥0.999,则仪器符合FAD认证要求。该认证方法可以保证紫外可见分光光度计性能正常,使测量结果准确、可靠。

Discussion of FDA Instruments Authentication Methods of Ultraviolet Visible Spectrophotometer

The method of the performance verification of the ultraviolet visible spectrophotometer in accordance with the FDA was introduced. The light energy,accuracy of absorbance and linearity were mainly certificated. At 235,257,313, 350 nm,the standard potassium dichromate solution was determined respectively. If the absorption coefficient were in the range of 123.0-126.0,142.8-146.2,47.0-50.3, 105.5-108.5, respectively, the instrument conformed to the FDA authentication requirements. Potassium dichromate standard solution was determined,if the linear correlation coefficient of absorbance and the concentrationsr2≥0.999,the instrument conformed to the requirements. The verification method can ensure the performance of the ultraviolet visible spectrophotometer at normal level,and obtain the accurate and reliable results.