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Forensic application of the luminol reaction as a presumptive test for latent blood detection
Authors:Filippo Barni  Simon W. Lewis  Gordon M. Miskelly
Affiliation:a Molecular Biology and Genetics Unit, Carabinieri Scientific Investigation Department of Rome, Viale di Tor di Quinto 119, 00191 Rome, Italy
b Department of Applied Chemistry, Curtin University of Technology, G.P.O. Box U1987 Perth, Western Australia 6845, Australia
c Department of Chemistry, The University of Auckland, Private Bag 92019, Auckland, New Zealand
Abstract:
The forensic application of the luminol chemiluminescence reaction is reviewed. Luminol has been effectively employed for more than 40 years for the presumptive detection of bloodstains which are hidden from the naked eye at crime scenes and, for this reason, has been considered one of the most important and well-known assays in the field of forensic sciences. This review provides an historical overview of the forensic use of luminol, and the current understanding of the reaction mechanism with particular reference to the catalysis by blood. Operational use of the luminol reaction, including issues with interferences and the effect of the luminol reaction on subsequent serological and DNA testing is also discussed.
Keywords:Luminol chemistry   Chemiluminescence   Forensic   Latent bloodstains   DNA typing
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