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A surface plasmon resonance biosensor for detecting Pseudomonas aeruginosa cells with self-assembled chitosan-alginate multilayers |
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| Authors: | Jung-Soon Park Chang-Moon Lee Ki-Young Lee |
| Institution: | a Department of Material and Biochemical Engineering, Chonnam National University, Gwangju 500-757, South Korea b Department of Nuclear Medicine & Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, Jeonbuk 561-712, South Korea c Department of Applied Chemical Engineering & The Research Institute for Catalysis, Chonnam National University, Gwangju 500-757, South Korea |
| Abstract: | A self-assembled multilayer (SAMu) including the alginate layer was prepared for detecting Pseudomonas aeruginosa cells in a solution and its potential was evaluated with a BIAcore system. After layer-by-layer formation, the refractive units (RU) values monitored with the biosensor increased by the interaction between the layers. The responses by the binding of P. aeruginosa cells to the alginate-immobilized SAMu were visualized immediately upon injection of the cell suspension. The RU values after injection of the cells were measured with approximately 1152, 656 and 173 for 1 × 109, 1 × 108 and 1 × 107 CFU/ml. This result suggests that the alginate-immobilized SAMu will have useful application for detecting P. aeruginosa cells in a biosensor analysis. |
| Keywords: | Biosensor Chitosan Alginate Pseudomonas aeruginosa Self-assembled multilayers Pathogen detection |
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