Direct electrochemistry and electrocatalysis of hemoglobin in gelatine film modified glassy carbon electrode |
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| Authors: | Hui Yao Nan Li Jun-Jie Zhu |
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| Institution: | a Key Lab. of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China
b College of Applied Chemistry, Shenyang Institute of Chemical Technology, Shenyang 110142, PR China |
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| Abstract: | Direct electrochemical and electrocatalytic behavior of hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containing gelatine (Gel) films was investigated. The characteristics of Hb/Gel film modified GC electrode were performed by using SEM microscopy, UV-vis spectroscopy and electrochemical methods. The immobilized Hb showed a couple of quasi-reversible redox peak with a formal potential of −0.38 V (versus SCE) in 0.1 M pH 7.0 PBS. The formal potential changed linearly from pH 4.03 to 8.41 with a slope value of −52.0 mV pH−1, which suggested that a proton transfer was accompanied with each electron transfer (ET) in the electrochemical reaction. The Hb/gelatine/GCE displayed a rapid amperometric response to the reduction of H2O2 and nitrite. |
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| Keywords: | Hemoglobin Gelatine Coimmobilize Direct electron transfer Electrocatalysis Electrochemistry |
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