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Development of a new high performance low pressure chromatographic system using a multisyringe burette coupled to a chromatographic monolithic column |
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| Authors: | Hilda M González-San Miguel Víctor Cerdà-Martín |
| Institution: | a Department of Quality Assurance and Pharmaceutical Technology, Food and Pharmaceutical Sciences Institute, University of Havana, Cuba b Department of Analytical Chemistry, Faculty of Chemistry, University of Havana, Cuba c Department of Chemistry, University of the Balearic Islands, Spain |
| Abstract: | A novel combination of high performance low pressure chromatography with multisyringe flow injection analysis is presented. This system comprises a multisyringe module, three low pressure solenoid valves, a monolithic Chromolith Flash RP-18e column and a diode array spectrophotometer. UV detection is carried out at 250 nm. AutoAnalysis software is used for instrumental control and automated data collection. The results obtained with multisyringe liquid chromatography (MSC) were compared with those obtained with a HPLC system using similar conditions. The chromatographic parameters were calculated from a mixture of anthracene and thiourea using a mobile phase containing acetonitrile-water (60:40) at a flow rate of 2 ml min−1. The proposed MSC system has been successfully applied to the determination of amoxicillin, ampicillin and cephalexin using a mobile phase of sodium acetate buffer (pH 6.2, 0.1 mol l−1)-methanol (90:10) at a flow rate of 2 ml min−1. The low-cost, flexibility and simplicity of MSC should be highlighted. |
| Keywords: | Multisyringe liquid chromatography MSC Monolithic column Amoxicillin Ampicillin Cephalexin |
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