以氨基酸为配体的血液灌流用内毒素吸附剂的制备及性能研究

以琼脂凝胶为载体,氨基酸为配体,合成了内毒素吸附剂,并对其吸附效果进行了比较.对吸附剂的各种性质进行了表征,选取琼脂-赖氨酸吸附剂对内毒素血症的兔子进行血液灌流,可将血浆中的内毒素降至接近正常值,最高清除率达73.6%,且吸附剂具有良好的血液相容性.

Preparation of Adsorbents for Endotoxin Removal in Hemoperfusion with Amino Acid as the Ligand and Investigations on Their Properties

Endotoxermia is caused by endotoxin, lipopolysaccharides(LPS) released from gram-negative bacteria cell wall. Endotoxin is composed of a hydrophilic polysaccharide moiety and a hydrophobic lipid moiety(lipid A) that anchors the molecules in the outer membrane. The sugar residues may be partially phosphorylated and serious endotoxin intoxication can cause sepsis and septic shocks, leading to severe hypertension, cardiovascular collapse, multiple organ failure and death. Removal of endotoxin duly and effectually from patients' blood is very important in clinic. In this paper, with agar gel as the carrier and amino acids as the ligands, adsorbents for endotoxin were prepared and their adsorption capacity for endotoxin were compared. The properties of adsorbents were investigated. The adsorbent with L-lysine as the ligand was chosen to be used for hemoperfusion. The adsorbent can remove plasma endotoxin of endotoxemia rabbit model to close to the normal level and the highest clearance percentage reached 73.6%. The adsorbent has a good blood compatibility.