Sensitive HPLC-fluorometric and HPLC-MS determination of diarrhetic shellfish poisoning (SP)-toxins as 4-bromomethyl-7-methoxycoumarin esters |
| |
| Authors: | Jin-Lin Shen Christian Hummert B Luckas |
| |
| Institution: | (1) Peking University, Department of Chemistry, Laboratory of Bioorganic Chemistry, Beijing 100871, P.R. China, CN;(2) University of Jena, Institute of Nutrition and Environment, Dornburger Strasse 25a, D-07743 Jena, Germany, DE |
| |
| Abstract: | Extracts containing the diarrhetic shellfish poisoning (DSP) toxins okadaic acid (OA), dinophysistoxin-2 (DTX2), and dinophysistoxin-1 (DTX1) were purified on a silica gel cartridge and derivatized with 4-bromomethyl-7 methoxycoumarin (BrMmc). After pre-column derivatization
the BrMmc derivatives of the DSP toxins were directly injected into an HPLC system, isocratically eluted, and quantified by
fluorescence detection. The signals of the esters showed good linearity in the fluorescence detector within the examined contamination
range of 0.03 mg DSP/kg to 2.5 mg DSP/kg. The detection limits for the DSP toxins as 7-Mmc esters were 0.04 ng (corresponding
to 0.05 mg DSP/kg). The chromatographic conditions allow to couple the HPLC device with mass spectrometry. The method was
tested with various mussel tissue samples.
Received: 14 December 1995/Revised: 26 January 1996/Accepted: 30 January 1996 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
