| MOLECULAR CLONING OF A NEW VARIANT OF HUMAN PAPILLOMAVIRUS TYPE 6 ISOLATED FROM A CHINESE WOMAN AND EXPRESSION OF ITS L1 GENE IN E. coli——MOLECULAR CLONING & EXPRESSION OF HPV6 GENE |
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| 引用本文： | 舒琍琍,冯慧敏,金奇,侯云德.MOLECULAR CLONING OF A NEW VARIANT OF HUMAN PAPILLOMAVIRUS TYPE 6 ISOLATED FROM A CHINESE WOMAN AND EXPRESSION OF ITS L1 GENE IN E. coli——MOLECULAR CLONING & EXPRESSION OF HPV6 GENE[J].中国科学B辑(英文版),1992(5). |
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| 作者姓名： | 舒琍琍 冯慧敏 金奇 侯云德 |
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| 作者单位： | National Laboratory of Molecular Virology and Genetic Engineering，Beijing 100052，PRC，National Laboratory of Molecular Virology and Genetic Engineering，Beijing 100052，PRC，National Laboratory of Molecular Virology and Genetic Engineering，Beijing 100052，PRC，National Laboratory of Molecular Virology and Genetic Engineering，Beijing 100052，PRC Department of Microbiology and Immunology，Sun Yat-sen University of Medical Sciences，Guangzhou 510089.，Department of Microbiology and Immunology，Sun Yat-sen University of Medical Sciences，Guangzhou 510089. |
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| 摘 要： | A human papillomavirus genome DNA of 7.9 kb from a Chinese woman with genital condyloma acuminata was cloned in Bam HI site of pAT153. According to the results obtained from Southern blotting, restriction mapping as well as partial DNA sequencing, the isolated genome (HPV6BV) had obvious variance and was referred to as a new variant of HPV6 found in China the first time. HPV6BV L1 gene was successfully expressed in E. coli as a fusion protein with pUR288. The β-galactosidase/L1 fusion protein reacted with both β-galactosidase antiserum and HPV antibody using Western blot technique. The E. coli-produced fusion protein, possessing HPV antigenicity, may provide a reagent for clinical diagnosis and epidemiological survey.
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