Biopolymer Synthesized by Strains of Xanthomonas sp Isolate from Brazil Using Biodiesel-Waste

The future supplies and usage of glycerol are expected to increase as biodiesel plants increase production, and the output will greatly outpace demand. Biodiesel production has already had a significant impact on the price of refined glycerol. A major concern of glycerol producers is the reduced price of glycerol resulting from the increased production of biodiesel. Some alternative uses for this glycerol that have been investigated are substrates for fermentation process or the production of biosurfactants, fatty acids, biopolymers, and others products. This work had as objective to evaluate two strains of Xanthomonas sp isolate from Brazil for xanthan gum in orbital agitator, analizing the apparent viscosity of aqueous solutions and selecting viscosity. The experiments of xanthan gum production were realized in orbital agitator with 120 rpm agitation, for cells production, and 180rpm, for biopolymer production, under a 28 °C temperature. The rheology of the fermentation broth was analyzed by apparent viscosity and the polymer was recovered with ethanol (1:3, v/v). After its recovery, the productivity evaluation was performed. The productivity were 0.157 and 0.363 gL^?1 for C1 and 0.186 and 0.363 gL^?1 for C9 to media glycerol or glycerol and sucrose, respectively. The viscosity analysis was performed for aqueous solutions 3%, at 25 °C, the best apparent viscosity was obtained using gum synthesized with glycerol and sucrose (50:50) at 25 °C, 143 mPa.s^?1 from Xanthomonas sp C1.