LC–IT–TOF MS法分析湖南黑茶中水溶性成分

建立了湖南黑茶中水溶性成分的液相色谱–离子阱串联飞行时间质谱(LC–IT–TOF MS)快速分离和确证方法。实验用90℃热水恒温提取湖南黑茶样品中水溶性成分,采用液相色谱–离子阱串联飞行时间质谱仪对其中的水溶性提取物进行分离测定。利用准确质量数匹配和自建质谱数据库检索,结合保留时间、多级质谱、特征碎片离子等信息,对湖南黑茶中的43种水溶性化合物的进行了初步确证。该法可得到黑茶独特滋味品质的特征成分,为湖南黑茶品质评价和质量控制提供了一种新的技术手段。

Determination of Water-Soluble Compounds in Black Tea by LC-IT-TOF MS Method

The liquid chromatography-ion trap time of flight mass spectrometry (LC-IT-TOF MS)method for rapid separation and identification of water-soluble components in Hunan dark tea was established. The samples of Hunan dark tea were extracted by hot water at 90℃ . The water-soluble extracts were analyzed by liquid chromatography-ion trap time of flight mass spectrometry (LC-IT-TOF MS). The 43 compounds were isolated and identified simultaneously by using accurate mass matching and self-established mass spectrometry database combined with retention time,multiple-stage ion mass spectral,characteristic fragment ions. Through identification of the characteristic component of dark tea's unique taste quality, a new technical means for Hunan dark tea quality evaluation and control was provided.