A Highly Sensitive Aptamer-Nanogold Catalytic Resonance Scattering Spectral Assay for Melamine |
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Authors: | Aihui Liang Lianping Zhou Huimin Qin Yi Zhang Huixiang Ouyang Zhiliang Jiang |
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Institution: | (1) Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, the Ministry of Education, Guangxi Normal University, Guilin, 541004, China; |
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Abstract: | The aptamer (ssDNA) was used to label nanogold (NG) particle to fabricate an aptamer–nanogold (NGssDNA) probe for melamine.
The probe was stabile in pH 6.6 Na2HPO4–NaH2PO4 buffer solutions and in the presence of high concentration of electrolyte. Upon addition of melamine, it interacted with
the probe to form big NGssDNA-melamine aggregations that led to the resonance Rayleigh scattering (RRS) intensity at 566 nm
increased greatly. The increased RRS intensity (ΔI) is linear to melamine concentration in the range of 1.89–81.98 μg/L, with
a detection limit of 0.98 μg/L melamine. The unreacted probe in the aptamer reaction solution exhibited strong catalytic effect
on the slow Cu2O particle reaction between glucose and Fehling reagent, but the catalytic activity of NG aggregations is very weak. When
melamine concentration increased, the unreacted probe decreased, the RRS peak intensity at 614 nm decreased. The decreased
RS intensity is linear to melamine concentration in the range of 0.63–47.30 ng/L melamine, with a detection limit of 0.38 ng/L.
The aptamer-modified nanogold catalytic RRS assay was applied to determination of melamine in milk, with high sensitivity
and selectivity, simplicity and rapidity. |
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