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Characterization of membrane proteins in isolated native cellular membranes by dynamic nuclear polarization solid-state NMR spectroscopy without purification and reconstitution |
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| Authors: | Jacso Tomas Franks W Trent Rose Honor Fink Uwe Broecker Jana Keller Sandro Oschkinat Hartmut Reif Bernd |
| Affiliation: | 1. Helmholtz‐Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Ingolst?dter Landstrasse 1, 85764 Neuherberg (Germany);2. Munich Center for Integrated Protein Science (CIPS‐M) at Department Chemie, Technische Universit?t München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany);3. Leibniz‐Institut für Molekulare Pharmakologie (FMP), Robert‐R?ssle‐Strasse 10, 13125 Berlin (Germany);4. University of Kaiserslautern, Erwin‐Schr?dinger‐Strasse 13, 67663 Kaiserslautern (Germany) |
| Abstract: | Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid-state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivity to observe the membrane protein of interest. |
| Keywords: | membrane proteins NMR spectroscopy structural biology structure elucidation |
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