Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh2 on Hep-A-22 Cells |
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摘 要: | <正>A modified method of preparing 20(S)-ginsenoside Rh_2(G-Rh_2) and the inhibitory effect of 20(S)-ginse-noside Rh_2 on Hep-A-22 cells were investigated.The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure,and the degradation was performed at a high temperature.After G-Rh_2 had been isolated and purified,MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh_2 on the cells viability and morphological changes were observed.It was shown that 20(S)-ginsenoside Rh_2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage,membrane blebbing,chromosomal condensations,especially under the higher concentrations of it.In conclusion,20(S)-ginsenoside Rh_2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.
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关 键 词: | 人参皂甙 细胞活力 喉癌 制备 形态学变化 修改方法 抑制作用 大气压力 |
Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh2 on Hep-A22 Cells |
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Authors: | ZHOU Hong-yu JIN Yong-ri WEI Wei SHI Xiao-lei YANG Rui-jie YANG Shi-jie LI Xu-wen |
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Institution: | [1]College of Chemistry, Jilin Uuniversity, Changchun 130012, P. R. China [2]Basic Medical School Jilin Uuniversity, Changchun 130021, P. R. China |
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