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Single-molecule force spectroscopy of a protein globule covalently bound to a calcite surface |
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| Authors: | N. N. Denisov L. A. Shcheglova S. K. Sekatskii G. Dietler V. A. Nadtochenko |
| Affiliation: | (1) Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia;(2) Moscow Institute (University) of Physics and Technology, Dolgoprudnyi, Moscow oblast, 141700, Russia;(3) Laboratoire de Physique de large Matière Vivante, IPMC, BSP, Ecole Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland |
| Abstract: | Two methods of covalent grafting of a protein onto a calcite surface were developed. Single-molecule force spectroscopy was used to examine the characteristics of individual albumin-albumin polyclonal body complexes. The practical applicability of the proposed method of covalent grafting of a protein to a calcite surface was demonstrated. |
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