A Gas Chromatography-Molecular Rotational Resonance Spectroscopy Based System of Singular Specificity |
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Authors: | Prof Daniel W Armstrong Dr Mohsen Talebi Nimisha Thakur Dr M Farooq Wahab Dr Alexander V Mikhonin Matt T Muckle Dr Justin L Neill |
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Institution: | 1. Department of Chemistry & Biochemistry, University of Texas at Arlington, Arlington, TX, 76019 USA;2. AZYP, LLC, Arlington, TX, 76012 USA;3. BrightSpec, Inc., Charlottesville, VA, 22903 USA |
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Abstract: | We designed and demonstrated the unique abilities of the first gas chromatography–molecular rotational resonance spectrometer (GC-MRR). While broadly and routinely applicable, its capabilities can exceed those of high-resolution MS and NMR spectroscopy in terms of selectivity, resolution, and compound identification. A series of 24 isotopologues and isotopomers of five organic compounds are separated, identified, and quantified in a single run. Natural isotopic abundances of mixtures of compounds containing chlorine, bromine, and sulfur heteroatoms are easily determined. MRR detection provides the added high specificity for these selective gas-phase separations. GC-MRR is shown to be ideal for compound-specific isotope analysis (CSIA). Different bacterial cultures and groundwater were shown to have contrasting isotopic selectivities for common organic compounds. The ease of such GC-MRR measurements may initiate a new era in biosynthetic/degradation and geochemical isotopic compound studies. |
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Keywords: | Biologischer Abbau Isotopologen/Isotopomere Massenspektrometrie Rotationsspektroskopie Verbindungsspezifische Isotopenanalyse |
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