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Amphiphilic Polyphenylene Dendron Conjugates for Surface Remodeling of Adenovirus 5 |
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| Authors: | Jessica Wagner Longjie Li Dr Johanna Simon Dr Lea Krutzke Prof Dr Katharina Landfester Prof Dr Volker Mailänder Prof Dr Klaus Müllen Dr David Y W Ng Prof Dr Yuzhou Wu Prof Dr Tanja Weil |
| Institution: | 1. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
Graduate School Materials Science in Mainz, Staudingerweg 9, 55128 Mainz, Germany;2. Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, 430074 Wuhan, China;3. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany Department of Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstr. 1, 55131 Mainz, Germany;4. University Ulinic, Department of Gene Therapy, Helmholtzstr. 8/1, 89081 Ulm, Germany;5. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany |
| Abstract: | Amphiphilic surface groups play an important role in many biological processes. The synthesis of amphiphilic polyphenylene dendrimer branches (dendrons), providing alternating hydrophilic and lipophilic surface groups and one reactive ethynyl group at the core is reported. The amphiphilic surface groups serve as biorecognition units that bind to the surface of adenovirus 5 (Ad5), which is a common vector in gene therapy. The Ad5/dendron complexes showed high gene transduction efficiencies in coxsackie-adenovirus receptor (CAR)-negative cells. Moreover, the dendrons offer incorporation of new functions at the dendron core by in situ post-modifications, even when bound to the Ad5 surface. Surfaces coated with these dendrons were analyzed for their blood-protein binding capacity, which is essential to predict their performance in the blood stream. A new platform for introducing bioactive groups to the Ad5 surface without chemically modifying the virus particles is provided. |
| Keywords: | Amphiphile Dendrimere Gentechnik Proteine Viren |