Protein-Controlled Actuation of Dynamic Nucleic Acid Networks by Using Synthetic DNA Translators** |
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Authors: | Dr Alessandro Bertucci Dr Alessandro Porchetta Dr Erica Del Grosso Dr Tania Patiño Dr Andrea Idili Prof Francesco Ricci |
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Institution: | 1. Department of Chemistry, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy;2. Catalan Institute of Nanoscience and Nanotechnology (ICN2), Campus UAB, Bellaterra, 08193 Barcelona, Spain |
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Abstract: | Integrating dynamic DNA nanotechnology with protein-controlled actuation will expand our ability to process molecular information. We have developed a strategy to actuate strand displacement reactions using DNA-binding proteins by engineering synthetic DNA translators that convert specific protein-binding events into trigger inputs through a programmed conformational change. We have constructed synthetic DNA networks responsive to two different DNA-binding proteins, TATA-binding protein and Myc-Max, and demonstrated multi-input activation of strand displacement reactions. We achieved protein-controlled regulation of a synthetic RNA and of an enzyme through artificial DNA-based communication, showing the potential of our molecular system in performing further programmable tasks. |
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Keywords: | aptamers DNA strand displacement DNA-binding proteins dynamic DNA nanotechnology synthetic biology |
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