利用拉曼光谱分析顺铂诱导的胃癌细胞凋亡

利用激光拉曼光谱对顺铂诱导的胃癌细胞凋亡进行分析,胃癌细胞SGC7901经10μg·mL-1顺铂处理24,48,72 h后,一部分用于荧光染色,另一部分用扫描方式收集细胞的拉曼光谱.光谱结果依次经背景扣除、平滑、归一化、基线校正、峰拟合等方法预处理.荧光染色结果显示对照组细胞核染色均匀,而药物处理72 h后,核碎裂,呈致密浓染.拉曼光谱结果显示顺铂处理24,48,72 h后,与核酸及蛋白质相关的峰均有降低,其中783,1 002,1 343 cm-1峰72 h后依次降低为初始值的52%,64%,76%,表明顺铂能够诱导胃癌细胞凋亡,凋亡细胞内核酸和蛋白质含量降低.以上结果说明,拉曼光谱能提供生物细胞内物质变化的丰富信息,是实时检测细胞凋亡过程的有效手段.

Using Raman Spectroscopy to Analyze Apoptosis of Gastric Cancer Cells Induced by Cisplatin

Apoptosis of gastric cancer cells induced by cisplatin was investigated using laser Raman spectroscopy. Gastric cancer cells (SGC7901) were treated with 10 μg·mL-1 cisplatin for 24, 48 and 72 hours, then were divided into two parts, one for fluorescence staining, the other for collection of Raman spectra by means of scanning. The acquired spectra were then preprocessed by background elimination, smoothing, normalization, baseline correction, and peak fitting. Fluorescence staining result showed that the nucleuses from untreated group were uniformly stained, while those from the group treated for 72 hours were densely stained and broken. The spectra results revealed that the intensity of peaks associated with nucleic acid and protein decreased after the cells were incubated with cisplatin for 24, 48 and 72 hours. The intensity of peaks at 783, 1 002 and 1 343 cm-1 respectively fell to 52, 64 and 76 percent of the original value after 72 h of treatment, which indicated that cisplatin could induce apoptosis of gastric cancer cells and reduce the amount of nucleic acid and protein in the cells. The above results suggest that Raman spectra can provide abundant information about the changes in materials in cells and serve as an effective method for real time measurement of apoptosis.