Comparison of Hair Fatty Alcohols by N‐Alkylpyridinium Isotope Quaternization and Matrix‐assisted Laser Desorption/ionization Mass Spectrometry for Drug Abuse Monitoring |
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| Authors: | Hang Wang Haoyang Wang Li Zhang Jing Zhang Xianyi Zhuo Yi Huang Yinlong Guo |
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| Institution: | 1. Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;2. Institute of Forensic Science, Ministry of Justice, Shanghai Key Laboratory of Forensic Medicine, Shanghai 200063, China |
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| Abstract: | Based on our previous report on N‐alkylpyridinium isotope quaternization (NAPIQ) for the analysis of alcoholic and α,β‐unsaturated ketone compounds, we have further applied NAPIQ method in the screening of hair lipids in drug abusers. Relative isotopic quantification was used for comparison of fatty alcohols between normal and drug abuse group. The NAPIQ strategy was proven to be a high‐throughput method in the metabolic comparison studies of different group samples. The attached N‐cationic pyridinium significantly improved the detection sensitivity for these fatty alcohols in matrix‐assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric (MALDI‐FTMS) analysis. The experimental results showed that the levels of fatty alcohols in the hair of heroin abuse group decreased significantly compared with the normal groups, which may be the results of the inducing of peroxidation enzyme. NAPIQ was proven to be an effective and alternative method in the research of fatty alcoholic metabolism for drug abuse monitoring. |
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| Keywords: | pyridinium quaternization MALDI mass spectrometry fatty alcohols drug abuse |
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