丹参酮ⅡA等中草药有效成分与白蛋白结合反应的光谱研究

在β－环糊精存在的缓冲体系中，利用光谱法研究了丹参酮ⅡA等有效成分与人血清蛋白(HSA)折结合反应。结果表明：丹参酮ⅡA等与HSA结合后，紫外吸收有红移现象，且吸收强度增加；它们对HSA的荧光有较强的猝灭作用。根据Lineweaver-Burk双倒数图，求得了它们与HSA的解离常数，基于Foerster无辐射能量转移机理，确定了它们的第一结合部位与HSA中214位色氨酸残基的距离。

Spectroscopic Studies on the Binding of Effective Component of Chinese Herbs with Human Serum Albumin

Tanshione II A and Cryptotanshinone are the main effective components of the traditional Chinese medicine Salvia miltiorrhiza bung. They both have many kinds of physiologic activities. When a medicine enters the body, it always goes to the acceptor site and has pharmacodynamic action by way of storage and carriage of plasma. Serum albumin is the major serum protein, which can combine with many inner and outer source compounds. In this paper, we have studied the interaction of Tanshinone with the human serum albumin(HSA) in beta-CD buffer system by spectroscopic method. The results show that the absorption maximum of Tanshinone exhibit red shift upon binding to HSA and the absorbance enhance. Tanshinone have stronger fluorescence quenching effect on HSA and the dissociation constants of Tanshinone bound to HSA are calculated. The distance of protein Trp-214 to the first binding site is also calculated on the basis of energy transfer between HSA and bound Tanshinone.