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GC-MS and LC-MS Identification of the Phenolic Compounds Present in the ethyl Acetate Fraction Obtained from Senna tora,L. Roxb. seeds
Authors:Noha Fathalla  Mokhtar Bishr  Abdel Nasser Singab  Osama Salama
Institution:1. Faculty of Pharmaceutical sciences and pharmaceutical industries, Future University in Egypt, Cairo, Egypt;2. Arab Company for Pharmaceuticals and Medicinal Plants, El-Sharkya, Egypt;3. Faculty of Pharmacy, Ein Shams University, Cairo, Egypt
Abstract:The aim of this work is to characterize the active constituents present in the ethyl acetate fraction of Senna tora, L. Roxb. seeds. Due to the fact that the main biological activity of S. tora, L seeds is attributed to its phenolic compounds which are mainly isolated from Ethyl acetate fraction, to avoid repetition of work and to save time, it was deemed necessary to confirm the identity of these phenolic compounds. This was done by GC-MS and LC-MS analysis of the ethyl acetate fraction where the structures of the isolated compounds were established on the basis of molecular ion peak and their fragmentation pattern. They were identified as Chrysophanol, Chrysarobin, 10-hydroxy-5-methoxy-2-methyl-1, 4-anthracenedione, Rubrofusarin, Parietin, Griseoxanthone-B, Isotorachrysone, and Cumbiasin B.

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Keywords:Senna tora L  Roxb    ethyl acetate fraction  GC-MS  LC-MS  phenolic compounds  anthraquinones
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